Publications

Publications

* equal contribution, # corresponding authors

2019

 

An experimental assay of the interactions of amino acids from orthologous sequences shaping a complex fitness landscape
Victoria O. Pokusaeva*, Dinara R. Usmanova*, Ekaterina V. Putintseva*, Lorena M. Espinar Calvo, Karen S. Sarkisyan, Alexander S. Mishin, Natalya S. Bogatyreva, Dmitry N. Ivankov, Arseniy V. Akopyan, Sergey Ya. Avvakumov, Inna S. Povolotskaya, Guillaume J. Filion, Lucas B. Carey#, Fyodor A. Kondrashov#, PLOS Genetics, 2019.

A brief review of bioluminescent systems (2019)
Aubin Fleiss and Karen S. Sarkisyan#, Current Genetics, 2019.


2018

A genetically encodable bioluminescent system from fungi
Alexey Kotlobay*, Karen S. Sarkisyan*#, Yuliana Mokrushina*, Marina Marcet-Houben*, Ekaterina O. Serebrovskaya*, Nadezhda M. Markina, Andrei Y. Gorokhovatsky, Konstantin V. Purtov, Valentin N. Petushkov, Natalja S. Rodionova, Tatiana V. Chepurnykh, Liliia I. Fakhranurova, Louisa Gonzalez Somermeyer, Elena B. Guglya, Rustam Ziganshin, Aleksandra S. Tsarkova, Zinaida M. Kaskova, Victoria Shender, Maksim Abakumov, Tatiana Abakumova, Inna S. Povolotskaya, Fyodor Eroshkin, Andrey Zaraisky, Alexander S. Mishin, Sergey V. Dolgov, Maria D. Logacheva, Nikolay Zolotarev, Eugene P. Kopantzev, Hans Waldenmaier, Anderson G. Oliveira, Yuichi Oba, Ekaterina Barsova, Ekaterina A. Bogdanova, Toni Gabaldón, Cassius V. Stevani, Ivan V. Smirnov, Josef I. Gitelson, Sergey Lukyanov, Fyodor A. Kondrashov, and Ilia V. Yampolsky#, PNAS, 2018.
For more information, see project description


2017

Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal
Nina G. Bozhanova, Mikhail S. Baranov, Natalia V. Klementieva, Alexey S. Gavrikov, Karen S. Sarkisyan, Ilia V. Yampolsky, Elena V. Zagaynova, Sergey A. Lukyanov, Konstantin A. Lukyanov, Alexander S. Mishin. Chemical Science, 2017, 8, 7138-7142.

Yellow and orange fluorescent proteins with tryptophan-based chromophores
Nina G. Bozhanova, Mikhail S. Baranov, Karen S. Sarkisyan, Roman Gritсenko, Konstantin V. Mineev, Svetlana V. Golodukhina, Nadezhda S. Baleeva, Konstantin A. Lukyanov and Alexander S. Mishin. ACS Chemical Biology, 2017, doi: 10.1021/acschembio.7b00337.


2016

Local fitness landscape of the green fluorescent protein
Karen S. Sarkisyan*, Dmitry A. Bolotin*, Margarita V. Meer, Dinara R. Usmanova, Alexander S. Mishin, George V. Sharonov, Dmitry N. Ivankov, Nina G. Bozhanova, Mikhail S. Baranov, Onuralp Soylemez, Natalya S. Bogatyreva, Peter K. Vlasov, Evgeny S. Egorov, Maria D. Logacheva, Alexey S. Kondrashov, Dmitry M. Chudakov, Ekaterina V. Putintseva, Ilgar Z. Mamedov, Dan S. Tawfik, Konstantin A. Lukyanov, Fyodor A. Kondrashov. Nature, 2016, 533, 397–401.


2012 – 2015

KillerOrange, a genetically encoded photosensitizer activated by blue and green light
Karen S. Sarkisyan*, Olga A. Zlobovskaya*, Dmitry A. Gorbachev*, Nina G. Bozhanova, George V. Sharonov, Dmitry B. Staroverov, Eugeny S. Egorov, Anastasia V. Ryabova, Kyril M. Solntsev, Alexander S. Mishin, Konstantin A. Lukyanov. PLOS One, 2015, 10:e0145287

Green fluorescent protein with anionic tryptophan-based chromophore and long fluorescence lifetime
Karen S. Sarkisyan, Alexander S. Goryashchenko, Dmitry A. Gorbachev, Peter V. Lidsky, Nina G. Bozhanova, Andrey Yu. Gorokhovatsky, Alina R. Pereverzeva, Alina P. Ryumina, Victoria V. Zherdeva, Alexander S. Savitsky, Kyril M. Solntsev, George V. Sharonov, Konstantin A. Lukyanov, Alexander S. Mishin. Biophysical Journal, 2015, 109:380-389.

Structure of the green fluorescent protein NowGFP with an anionic tryptophan-based chromophore
Vladimir Z. Pletnev, Nadya V. Pletneva, Karen S. Sarkisyan, Alexander S. Mishin, Konstantin A. Lukyanov, Ekaterina A. Goryacheva, Rustam H. Ziganshin, Zbigniew Dauter and Sergei Pletnev. Acta Crystallographica D, 2015, 71:1699-1707

Crystal structure of phototoxic orange fluorescent proteins with a tryptophan-based chromophore
Nadya V. Pletneva, Vladimir Z. Pletnev, Karen S. Sarkisyan, Dmitry A. Gorbachev, Evgeny S. Egorov, Alexander S. Mishin, Konstantin A. Lukyanov, Zbigniew Dauter, Sergei Pletnev. PLOS One, 2015, 10:e0145740. doi: 10.1371/journal.pone.0145740.

Fluorescent protein based FRET pairs with improved dynamic range for fluorescence lifetime measurements
Bobin George Abraham , Karen S. Sarkisyan, Alexander S. Mishin, Ville Santala, Nikolai V. Tkachenko, Matti Karp. PLOS One, 2015, 10:e0134436.

Infra-red fluorescent protein iRFP as an acceptor for Forster Resonance Energy Transfer
Olga A. Zlobovskaya, Karen S. Sarkisyan, Konstantin A. Lukyanov. Bioorganic Chemistry, 2015, 41:299-304.

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics
Jasper Akerboom, Nicole Carreras Calderón, Lin Tian, Sebastian Wabnig, Matthias Prigge, Johan Tolö, Andrew Gordus, Michael B. Orger, Kristen E. Severi, John J. Macklin, Ronak Patel, Stefan R. Pulver, Trevor J. Wardill, Elisabeth Fischer, Christina Schüler, Tsai-Wen Chen, Karen S. Sarkisyan, Jonathan S. Marvin, Cornelia I. Bargmann, Douglas S. Kim, Sebastian Kügler, Leon Lagnado, Peter Hegemann, Alexander Gottschalk, Eric R. Schreiter, Loren L. Looger. Frontiers in Molecular Neuroscience, 2013; 6:2.

Tryptophan-based chromophore in fluorescent proteins can be anionic
Karen S. Sarkisyan, Ilia V. Yampolsky, Kyril M. Solntsev, Sergey A. Lukyanov, Konstantin A. Lukyanov & Alexander S. Mishin. Scientific Reports, 2012; 2:608